A non-invasive Liquid biopsy-based approach for circulating cell-free RNA-sequencing and high-plex Proteomics to identify novel molecular biomarkers signatures for Sarcopenia

Sarcopenia is characterized by the age-related loss of skeletal muscle mass, regeneration, strength and function. It is most prevalent among the elderly population and is exacerbated among patients with Type 2 Diabetes mellitus. Sarcopenia is associated with muscle weakness, lack of mobility, difficulty walking, reduced stamina, falls, frailty and mortality. Currently, there are no effective treatments available, and the molecular mechanisms underlying its pathophysiology are poorly understood. Utilisation of non-invasive approaches such as liquid biopsies to identify new potential early-stage biomarkers and therapeutic targets to treat Sarcopenia is paramount. This study aims to examine the distinct expression patterns of a set of circulating miRNAs and circulating proteins in the serum/plasma of elderly patients with or without Sarcopenia. This analysis aims to assess the potential utility of these miRNAs and circulating proteins as biomarkers or in the exploration of therapeutic targets. Disease mechanisms will be investigated by the use of pharmacological inhibitors and knockdown studies in differentiated skeletal muscle myotubes cultured either as monocultures or 3D-bioprinting scaffolds. This research aims to identify novel biomarkers and therapeutic targets for Sarcopenia, ultimately improving the wellbeing of Sarcopenia patients. During this PhD studentship, the student will acquire essential experimental techniques including cell culturing, molecular pharmacology, RNA/protein isolation, RNA-sequencing (RNA-seq), microRNAs (miRNAs), proteomics, qRT-PCR, Western blotting and immunofluorescence. Previous experience in miRNAs and RNA-seq in liquid biopsies is desirable.

These PhD opportunities will include comprehensive personal and professional development training and a mentoring programme from the University of Westminster Graduate School. The researchers will join a School firmly committed to decolonising and diversifying policies, practices, and cultures within, and beyond, Higher Education. 

 Entry requirements and how to apply

Candidates should have a minimum classification of 2.1 in their bachelor’s degree or equivalent and preferably a Masters degree. Applicants whose secondary level education has not been conducted in the medium of English should also demonstrate evidence of appropriate English language proficiency normally defined as IELTS: 6.5 (overall score with not less than 6.0 in any of the individual elements).

You can read more about what should be in a PhD research proposal here:

https://www.westminster.ac.uk/study/postgraduate/research-degrees/entry-requirements/how-to-write-your-research-proposal

You can read more about our entry requirements here:

http://westminster.ac.uk/study/postgraduate/research-degrees/entry-requirements

Applications should be submitted by Friday 16^th May 2025. Interviews will take place in June.

It is essential you include the code of the project you are applying for in your personal statement i.e. “SLS SF1”.